LOCUS       40924_40407        4370 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector psiLentGene Neomycin, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Virdugirene J.
  TITLE     siLentGene U6 Hairpin Cloning Systems Technical Manual #TM247
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Virdugirene J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4370)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4370)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(140..158)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(176..192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(200..216)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(224..254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(269..290)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        465..822
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             863..1654
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1692..1740
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(1933..2521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2695..3552)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3553..3657)
                     /label=AmpR promoter
     rep_origin      complement(3735..4190)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4331..4347
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4354..4370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt
      121 ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
      181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgctgatctg cgcagcacca
      421 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
      481 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      541 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      601 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      661 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      721 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
      781 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac
      841 gctagcgatc gcccgggcca ccatgattga acaagatgga ttgcacgcag gttctccggc
      901 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
      961 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     1021 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     1081 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     1141 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     1201 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     1261 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     1321 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     1381 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     1441 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     1501 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     1561 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     1621 catcgccttc tatcgccttc ttgacgagtt cttctgactc gagtttaaac tctagaaccg
     1681 gtcatggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     1741 ttcgaactag aagcttgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1801 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1861 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1921 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1981 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2041 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2101 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2161 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2221 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2281 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2341 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2401 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2461 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2521 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2581 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2641 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2701 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2761 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2821 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2881 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2941 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3001 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3061 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3121 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3181 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3241 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3301 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3361 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3421 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3481 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3541 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3601 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3661 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3721 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3781 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3841 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3901 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3961 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4021 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4081 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4141 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     4201 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4261 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     4321 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
